Welcome!

This page is an outcome of an informal survey I made of several HPLC experts in preparation for an article I wrote for LCGC Magazine in 2017. The intention here is to collect a number of (mostly free) resources that users of liquid chromatography find very useful in their work on HPLC. If you know of an resource that you think is particularly useful but not listed here, I'd be happy to know about it. Please feel free to email me with suggestions.
~ Dwight R. Stoll (updated October, 2017)

Resources, Broadly Defined	Tutorials, Primers, and Guides	Tools for LC Simulation and Calculation
LCGC Website - Great free resource for all aspects of separation science John Dolan's LC Troubleshooting Bible - All of Dolan's LC Troubleshooting articles, searchable by keyword List of miscellaneous resources curated by LC Resources Analytical Sciences Digital Library - Collection of resources developed with funding from the U.S. National Science Foundation Agilent Teaching Resources for Academia - Collection of videos and animations demonstrating the principles of various LC and MS approaches	CHROMAcademy - Full learning management system of >1000 animated and video training courses, with customizable learning paths, and online troubleshooting tools, in chromatography, sample preparation, mass spectrometry, spectroscopy and basic laboratory skills. Constantly updated. Chromedia - Multimedia learning resources for sample preparation, chromatography, and mass spectrometry. Primers by Agilent List of all Primers Introduction to Liquid Chromatography Two-Dimensional Liquid Chromatography Primers by Waters List of all Primers Beginners Guide to HPLC Introductory Video about HPLC - Produced by the Royal Society of Chemistry, captures in 5 minutes the essence of how HPLC works and why it is so useful. Supelco Resources for Chiral Chromatography - multimedia learning resources for chiral separations	HPLC Simulator (www.multidlc.org/hplcsim/hplcsim.html) - Dynamic simulator for exploring the effects of operating variables on reversed-phase separations - Developed at Gustavus Adolphus College Web application (free) HPLC Simulator - More detailed simulator compared to hplcsim.html - Developed at U. Geneva Excel spreadsheet (free) HPLC Teaching Assistant - Calculators to facilitate teaching and learning about HPLC concepts - Developed at U. Geneva Excel spreadsheet (free) Calculator Apps for Parameter Estimation (e.g., pressure vs. flow rate) Agilent (free) (web, iOS) Method Transfer Tools - Calculators for scaling to different particle sizes, movement between instruments, etc. Thermo Excel spreadsheet (free) Waters Desktop application (free) Buffer Wizard - Web-based application designed to assist with calculations needed for preparing buffers (free and paid versions available)
Wall Charts and Quick-Start Guides	Tools for Column Selection and Characterization	Tools for Molecular Properties
LCGC's Sample Preparation Guide LCGC's LC Troubleshooting Guide	PQRI Website - Database of column parameters based on the Hydrophobic Subtraction (HS) model developed by Snyder, Dolan, and coworkers - maintained by United States Pharmacopoeia hplccolumns.org - Database of column parameters based on HS model, presented in a different way compared to USP PQRI site, and with unique tools for visualization - maintained by Stoll Laboratory Waters RP Column Selectivity Chart - Tool for comparing selectivities of different RP chemistries, and columns from different vendors ICOA Website - Classification tool for RP phases developed at Institut de Chimie Organique et Analytique	ChemSpider - Free chemical structure database providing access to millions of structures from hundreds of data sources. Enables predication of properties including pKa and log P using a number of different algorithms NIST WebBook - Free access to physical and chemical property data compiled by U.S. National Institute of Standards and Technology (NIST)